Nikon Eclipse LV 100D-U Microscope

Optical Microscopes

Location

MSE 250

Fees

Included in Fixed Fee-For-Service Membership

Instrument Specifications

Illumination Modes	Episcopic (EPI) Diascopic (DIA)
Microscopy Methods	EPI: Brightfield EPI: Darkfield EPI: Simplified Polarization EPI: Differential Interference Contrast (DIC) DIA: Brightfield
Objective Properties	Lateral Magnifications: 10x, 20x, 50x, 100x LU = Nikon Luminous Universal (Brightfield, Darkfield, DIC, and Polarized Light) Plan = Flat Field optical correction Fluor = Fluorite aberration correction Inf (symbol) = Infinity corrected tube length 0 = no coverglass correction (distortions if used with coverglass) BD = Brightfield or Darkfield A = DIC Prism setting WD = Working Distance in mm OFN = Field Number in mm
Camera	Canon Rebel T5 EOS DSLR
Image Capture Software	Canon EOS Utility

Useful Documentation

UCR training and operators manual	UCR MSE Nikon Training Notebook rev 3.3 (last modified May 20, 2021)
Images to establish scale bar using Image J	Scale Bar Images Zip
Nikon operators manual	Nikon LV-100D-U Manual

Nikon Training Videos

Nikon Training ver 1. (full playlist)	III. EPI Brightfield	VII. DIA Brightfield
0. Introduction	IV. EPI Darkfield	VIII. Image Capture
I. Microscope Layout	V. EPI Polarization	IX. Cleanup
II. Startup	VI. EPI Differential Interference Contrast	X. ImageJ